Recombinant Human TLR3 protein, His-Avi-tagged, Biotinylated

• Cat.No.: TLR3-1727H
• Product Overview: Biotinylated Recombinant Human TLR3 protein(Q6PCD4)(Ser23-Glu703) is expressed from HEK293 with His tag and Avi tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Ser23-Glu703
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Bio-activity: Immobilized Biotinylated Human TLR3, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.
• Molecular Mass: It has a predicted MW of 80.25 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC

Gene Information

• Official Symbol: TLR3
• Synonyms: TLR3; toll-like receptor 3; CD283; IIAE2
• Gene ID: 7098
• mRNA Refseq: NM_003265
• Protein Refseq: NP_003256
• MIM: 603029
• UniProt ID: O15455