Recombinant Human Thymidine Phosphorylase, His-tagged

• Cat.No.: TYMP-799H
• Product Overview: Recombinant human TYMP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 51.3 kDa (493aa), confirmed by MALDI-TOF.

Specification

• Cat. No.: TYMP-799H
• Description: Thymidine phosphorylase precursor, also known TYMP, is a platelet-derived endothelial cell growth factor that catalyzes the formation of thymine and 2-deoxy-D-ribose-1-phosphate from thymidine and orthophosphate. It is an angiogenic inducer that potently stimulates the growth of endothelial cells and induces chemotaxis. Increased expression of TYMP is seen in a wide variety of different solid tumors and inflammatory diseases and is often associated with poor prognosis.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAPPAPGDFS GEGSQGLPDP SPEPKQLPEL IRMKRDGGRL SEADIRGFVA AVVNGSAQGA QIGAMLMAIR LRGMDLEETS VLTQALAQSG QQLEWPEAWR QQLVDKHSTG GVGDKVSLVL APALAACGCK VPMISGRGLG HTGGTLDKLE SIPGFNVIQS PEQMQVLLDQ AGCCIVGQSE QLVPADGILY AARDVTATVD SLPLITASIL SKKLVEGLSA LVVDVKFGGA AVFPNQEQAR ELAKTLVGVG ASLGLRVAAA LTAMDKPLGR CVGHALEVEE ALLCMDGAGP PDLRDLVTTL GGALLWLSGH AGTQAQGAAR VAAALDDGSA LGRFERMLAA QGVDPGLARA LCSGSPAERR QLLPRAREQE ELLAPADGTV ELVRALPLAL VLHELGAGRS RAGEPLRLGV GAELLVDVGQ RLRRGTPWLR VHRDGPALSG PQSRALQEAL VLSDRAPFAA PSPFAELVLP PQQ
• Purity: > 90% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Protein length: 11-482 a.a.

Gene Information

• Gene Name: TYMP thymidine phosphorylase [ Homo sapiens ]
• Synonyms: TYMP; thymidine phosphorylase; TP; ECGF1; MNGIE; PDECGF; hPD-ECGF; endothelial cell growth factor 1 (platelet-derived); gliostatin; OTTHUMP00000196769; EC 2.4.2.4; TdRPase; Platelet-derived endothelial cell growth factor; PD-ECGF; OTTHUMP00000196768; TdRPase; Gliostatin
• Gene ID: 1890
• mRNA Refseq: NM_001113755
• Protein Refseq: NP_001107227
• MIM: 131222
• UniProt ID: P19971
• Chromosome Location: 22q13
• Pathway: Bladder cancer; Drug metabolism - other enzymes; Metabolic pathways; Pyrimidine metabolism
• Function: growth factor activity; platelet-derived growth factor receptor binding; thymidine phosphorylase activity