Recombinant Human Threonyl-TRNA Synthetase, His-tagged

• Cat.No.: TARS-1936H
• Product Overview: Recombinant humanTARS protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: TARS (Threonyl-tRNAsynthetase, cytoplasmic) belongs to the class-II aminoacyl-tRNA synthetasefamily. This protein has its main role in tRNA aminoacylation. The N-terminaldomain of the enzyme is responsible for the competition with the ribosomewhilst the catalytic and the C-terminal domain are involved in binding thetwo anticodon arm-like structures in the operator.
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150mM NaCl.
• Molecular Weight: 85.6kDa (743aa).
• Purity: > 90% by SDS -PAGE.
• Concentration: 0.5mg/ml (determinedby Bradford assay).
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MFEEKASSPS GKMGGEEKPI GAGEEKQKEG GKKKNKEGSG DGGRAELNPW PEYIYTRLEMYNILKAEHDS ILAEKAEKDS KPIKVTLPDG KQVDAESWKT TPYQIACGIS QGLADNTVIA KVNNVVWDLDRPLEEDCTLE LLKFEDEEAQ AVYWHSSAHI MGEAMERVYG GCLCYGPPIE NGFYYDMYLE EGGVSSNDFSSLEALCKKII KEKQAFERLE VKKETLLAMF KYNKFKCRIL NEKVNTPTTT VYRCGPLIDL CRGPHVRHTGKIKALKIHKN SSTYWEGKAD METLQRIYGI SFPDPKMLKE WEKFQEEAKN RDHRKIGRDQ ELYFFHELSPGSCFFLPKGA YIYNALIEFI RSEYRKRGFQ EVVTPNIFNS RLWMTSGHWQ HYSENMFSFE VEKELFALKPMNCPGHCLMF DHRPRSWREL PLRLADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCAME QIEDEIKGCLDFLRTVYSVF GFSFKLNLST RPEKFLGDIE VWDQAEKQLE NSLNEFGEKW ELNSGDGAFY GPKIDIQIKDAIGRYHQCAT IQLDFQLPIR FNLTYVSHDG DDKKRPVIVH RAILGSVERM IAILTENYGG KWPFWLSPRQVMVVPVGPTC DEYAQKVRQQ FHDAKFMADI DLDPGCTLNK KIRNAQLAQY NFILVVGEKE KISGTVNIRTRDNKVHGERT ISETIERLQQ LKEFRSKQAE EEF
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.
• OfficialSymbol: TARS
• Pathways: Aminoacyl-tRNAbiosynthesis; Cytosolic tRNA aminoacylation; Gene Expression; tRNAAminoacylation

Gene Information

• Gene Name: TARS threonyl-tRNA synthetase [ Homosapiens ]
• Synonyms: TARS; threonyl-tRNAsynthetase; ThrRS; threonyl-tRNA synthetase, cytoplasmic; threonine--tRNAligase; threonine tRNA ligase 1, cytoplasmic
• Gene ID: 6897
• mRNA Refseq: NM_152295
• Protein Refseq: NP_689508
• MIM: 187790
• UniProt ID: P26639
• Chromosome Location: 5p13.2
• Function: ATP binding; ligaseactivity; nucleotide binding; protein homodimerization activity;threonine-tRNA ligase activity