Recombinant Human TFF1 protein, His & GST-tagged

• Cat.No.: TFF1-1435H
• Product Overview: Recombinant Human TFF1 aa. (Met1~Phe84 (Accession # P04155)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Met1~Phe84
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Characteristic: The isoelectric point is 5.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: TFF1 trefoil factor 1 [ Homo sapiens (human) ]
• Official Symbol: TFF1
• Synonyms: pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21; breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; gastrointestinal trefoil protein pS2; polypeptide P1.A; protein pS2; trefoil factor 1
• Gene ID: 7031
• mRNA Refseq: NM_003225.2
• Protein Refseq: NP_003216.1
• UniProt ID: P04155