Recombinant Human TET2, GST-tagged

• Cat.No.: TET2-160H
• Product Overview: Recombinant human TET2 (1197-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1197-end a.a.
• Description: TET2 is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine which is involved in myelopoiesis. Defects in TET2 gene have been associated with several myeloproliferative disorders. TET2 play an important role in embryonic stem cell maintenance and inner cell mass cell specification. TET proteins have potential roles in epigenetic regulation through modification of 5mC to 5hmC. TET2 enzymatic activity is involved in myeloid tumorigenesis and this could be valuable as a diagnostic and prognostic tool for targeted therapies and to assess responses to anticancer drugs.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~124 kDa
• Purity: >70% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: TET2 tet methylcytosine dioxygenase 2 [ Homo sapiens ]
• Official Symbol: TET2
• Synonyms: TET2; tet methylcytosine dioxygenase 2; KIAA1546 , tet oncogene family member 2; methylcytosine dioxygenase TET2; FLJ20032; tet oncogene family member 2; probable methylcytosine dioxygenase TET2; MDS; KIAA1546; MGC125715
• Gene ID: 54790
• mRNA Refseq: NM_001127208
• Protein Refseq: NP_001120680
• MIM: 612839
• UniProt ID: Q6N021
• Chromosome Location: 4q24
• Function: metal ion binding; methylcytosine dioxygenase activity; methylcytosine dioxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen