Recombinant Human Testis-Specific Serine Kinase 1B, GST-tagged

• Cat.No.: TSSK1B-1113H
• Product Overview: Recombinant full-length human TSSK1B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Cat. No.: TSSK1B-1113H
• Description: TSSK1B (testis-specific serine kinase 1B) is a member of a unique subfamily of protein kinases belonging to the AMPK branch on the human kinome tree. TSSK1B is a serine/threonine kinases that is highly expressed in testis and lower express in pancreas, and weak expression in a few other tissues. TSSK members are present in the equatorial segment of human sperm. TSSK1B share 72% amino acid identity and 83% identity in the kinase domain with other family TSSK members and the TSSK1 gene is intronless. In vitro kinase assay shows phosphorylation of TSKS by TSSK1 and TSSK2.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 71kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50mM Tris-HCI, pH 7.5, 150mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1mM PMSF, 25% glycerol.
• Specific Activity: 60nmol/min/mg
• Purity: >80%
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.

Gene Information

• Gene Name: TSSK1B testis-specific serine kinase 1B [ Homo sapiens ]
• Official Symbol: TSSK1B
• Synonyms: TSSK1B; TSSK1; FKSG81; SPOGA4; STK22D; SPOGA1; STK22A; testis-specific serine/threonine-protein kinase 1; TSK-1; testis-specific serine kinase 1B; TSSK-1; testis-specific kinase 1; spermiogenesis associated 4; serine/threonine kinase FKSG81; testis-specific serine kinase 1; serine/threonine-protein kinase 22A; EC 2.7.11.1; EC 2.7.11serine/threonine kinase 22D (spermiogenesis associated)
• Gene ID: 83942
• mRNA Refseq: NM_032028
• Protein Refseq: NP_114417
• MIM: 610709
• UniProt ID: Q9BXA7
• Chromosome Location: 5q22.2
• Function: ATP binding; magnesium ion binding; nucleotide binding; protein serine/threonine kinase activity