Recombinant Human TEK Tyrosine Kinase, Endothelial, His-tagged

• Cat.No.: TEK-390H
• Product Overview: Recombinant human TEK(a.a. 771-end) with N-terminal His tag, MW=45 kDa, expressed in an Baculovirusinfected Sf9 cell expression system.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: 771-end a.a.
• Description: The TEK receptortyrosine kinase is expressed almost exclusively in endothelial cells in mice,rats, and humans. This receptor possesses a unique extracellular domaincontaining 2 immunoglobulin-like loops separated by 3 epidermal growth factor-likerepeats that are connected to 3 fibronectin type III-like repeats. The ligandfor the receptor is angiopoietin-1. Defects in TEK are associated withinherited venous malformations; the TEK signaling pathway appears to becritical for endothelial cell-smooth muscle cell communication in venousmorphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
• Molecule Weight: 45 kDa
• Form: 25 mM Tris-HCl, pH8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
• Purity: Greater than 90% bySDS-PAGE
• Specific Activity: 140 U/µg. One unitis defined as the amount of enzyme that will phosphorylate 1 pmol of Tyrsubstrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na-orthovanadate, 0.5 mM ATP, 30 µg/mlPoly (Glu:Tyr) 4:1 substrate, and 2 µg/ml recombinant Tie2.
• Applications: usefulfor the study of enzyme kinetics, screening inhibitors, and selectivityprofiling.
• Storage: >6 months at –80°C.
• OfficialSymbol: TEK
• Pathways: Angiogenesis;Angiopoietin receptor Tie2-mediated signaling; Cell surface interactions atthe vascular wall; Hemostasis; Rheumatoid arthritis; Tie2 Signaling

Gene Information

• Gene Name: TEK TEK tyrosine kinase,endothelial [ Homo sapiens ]
• Synonyms: TEK; TEK tyrosinekinase, endothelial; TIE2; VMCM; TIE-2; VMCM1; CD202B; angiopoietin-1receptor; hTIE2; p140 TEK; OTTHUMP00000021167; OTTHUMP00000227067; OTTHUMP00000227068;OTTHUMP00000227069; soluble TIE2 variant 1; soluble TIE2 variant 2;tyrosine-protein kinase receptor TEK; tunica interna endothelial cell kinase;tyrosine-protein kinase receptor TIE-2; EC 2.7.10.1
• Gene ID: 7010
• mRNA Refseq: NM_000459
• Protein Refseq: NP_000450
• MIM: 600221
• UniProt ID: Q02763
• Chromosome Location: 9p21
• Function: ATP binding;nucleotide binding; protein binding; protein kinase activity; proteintyrosine kinase activity; receptor activity; transmembrane receptor proteintyrosine kinase activity