Recombinant human TEA domain transcription factor 1 protein, His-tagged

• Cat.No.: TEAD1-02H
• Product Overview: Recombinant human TEA domain transcription factor 1 with N-terminal His6 fusion tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 246
• Description: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
• Form: Buffered aqueous solution
• Molecular Mass: 28.6 kDa
• AA Sequence: MHHHHHHLVPRGSGLNDIFEAQKIEWHERSIGTTKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVTSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRLVKD
• Purity: ＞90% by SDS-PAGE
• Applications: Antigen for antibody production
  ELISA
  WB
• Storage: Store it under sterile conditions at -20 to -80 ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.262 mg/ml
• Storage Buffer: Solution in 50mM Tris, 300mM NaCl, pH8.0, 0.1% SKL

Gene Information

• Gene Name: TEAD1
• Official Symbol: TEAD1
• Synonyms: AA; Atrophia areata peripapillary chorioretinal degeneration; NTEF 1; NTEF-1; NTEF1; REF1; SV40 transcriptional enhancer factor; TCF 13; TCF-13; TCF13; TEA domain family member
• Gene ID: 7003
• mRNA Refseq: NM_021961.6
• Protein Refseq: NP_068780.2
• UniProt ID: P28347