Recombinant Human TBC1 domain family, member 4 Protein, His&Flag&StrepII tagged

• Cat.No.: TBC1D4-065H
• Product Overview: Recombinant human TBC1D4/AS160 protein (O60343) (Gly 29-Lys 451) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: N-Twin strep and C-His&Flag
• ProteinLength: 29-451aa
• Description: This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Tag: N-Twin strep and C-His&Flag
• Molecular Mass: 53.3 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: TBC1D4 TBC1 domain family, member 4 [ Homo sapiens (human) ]
• Official Symbol: TBC1D4
• Synonyms: TBC1D4; TBC1 domain family, member 4; TBC1 domain family member 4; Akt substrate of 160 kDa; AS160; DKFZp779C0666; KIAA0603; akt substrate of 160 kDa; Acrg embryonic lethality minimal region ortholog; TBC (Tre-2, BUB2, CDC16) domain-containing protein
• Gene ID: 9882
• mRNA Refseq: NM_014832
• Protein Refseq: NP_055647
• MIM: 612465
• UniProt ID: O60343