Recombinant Human TAS1R3 HEK293T cell lysate

• Cat.No.: TAS1R3-18HCL
• Product Overview: Human TAS1R3 over-expression lysate was derived from Human HEK293T cell line.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene is a G-protein coupled receptor involved in taste responses. The encoded protein can form a heterodimeric receptor with TAS1R1 to elicit the umami taste response, or it can bind with TAS1R2 to form a receptor for the sweet taste response.
• Form: The cell lysate is provided in RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4).
• Molecular Mass: 93.3 kDa
• Usage: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
  1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Storage: The lysate is shipped with dry ice. Upon receiving, store the sample at -20centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20centigrade. Avoid repeated freeze-thaw cycles.
  Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: TAS1R3 taste receptor, type 1, member 3 [ Homo sapiens ]
• Official Symbol: TAS1R3
• Synonyms: TAS1R3; taste receptor, type 1, member 3; taste receptor type 1 member 3; T1R3; sweet taste receptor T1R3
• Gene ID: 83756
• mRNA Refseq: NM_152228
• Protein Refseq: NP_689414
• MIM: 605865
• UniProt ID: Q7RTX0
• Chromosome Location: 1p36
• Pathway: Carbohydrate digestion and absorption, organism-specific biosystem; Carbohydrate digestion and absorption, conserved biosystem; Class C/3 (Metabotropic glutamate/pheromone receptors), organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by GPCR, organism-specific biosystem; Taste transduction, organism-specific biosystem
• Function: protein heterodimerization activity; receptor activity; signal transducer activity; contributes_to sweet taste receptor activity; taste receptor activity; contributes_to taste receptor activity