Recombinant Human TANK, His-tagged

• Cat.No.: TANK-7244H
• Product Overview: Recombinant human TANK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• description: TANK, also known as TRAF interacting protein, is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby proposing that this protein is an inhibitor of TRAF function that regulates TRAF protein activity by sequestering TRAFs in a latent state in the cytoplasm. Overexpression of TANK inhibits TRAF2-mediated NF-Kappa-B activation signaled by CD40 and both TNF receptors and inhibits LMP1-mediated NF-kappa-B activation by blocking the association of TRAF2 with LMP1.
• source: E. coli
• species: Human
• tag: His
• form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol,2mM DTT
• molecular mass: 50.2 kDa (448aa)
• protein length: 1-425aa
• aa sequence: MGSSHHHHHH SSGLVPRGSH MGSMDKNIGE QLNKAYEAFR QACMDRDSAV KELQQKTENY EQRIREQQEQ LSLQQTIIDK LKSQLLLVNSTQDNNYGCVP LLEDSETRKN NLTLDQPQDK VISGIAREKL PKVRRQEVSS PRKETSARSL GSPLLHERGN IEKTFWDLKE EFHKICMLAK AQKDHLSKLN IPDTATETQC SVPIQCTDKT DKQEALFKPQ AKDDINRGAP SITSVTPRGL CRDEEDTSFE SLSKFNVKFP PMDNDSTFLHSTPERPGILS PATSEAVCQE KFNMEFRDNP GNFVKTEETL FEIQGIDPIA SAIQNLKTTD KTKPSNLVNT CIRTTLDRAA CLPPGDHNAL YVNSFPLLDP SDAPFPSLDS PGKAIRGPQQ PIWKPFPNQD SDSVVLSGTD SELHIPRVCE FCQAVFPPSI TSRGDFLRHL NSHFNGET
• purity: >85% by SDS - PAGE
• applications: SDS-PAGE
• storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• concentration: 0.5 mg/ml

Gene Information

• gene name: TANK TRAF family member-associated NFKB activator [ Homo sapiens ]
• official symbol: TANK
• synonyms: TANK; TRAF family member-associated NFKB activator; TRAF2; TRAF family member-associated NF-kappa-B activator; I TRAF; TRAF-interacting protein; I-TRAF
• Gene ID: 10010
• mrna refseq: NM_004180
• protein refseq: NP_004171
• mim: 603893
• uniprot id: Q92844
• chromosome location: 2q24-q31
• pathway: Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; RIG-I-like receptor signaling pathway, organism-specific biosystem; RIG-I-like receptor signaling pathway, conserved biosystem; RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways, organism-specific biosystem; TNF-alpha/NF-kB Signaling Pathway, organism-specific biosystem; TRAF6 mediated IRF7 activation, organism-specific biosystem
• function: metal ion binding; protein binding