Recombinant Human STAT3 protein, His-tagged

Cat.No. : STAT3-31H
Product Overview : Recombinant Human STAT3 protein(Ile576~Pro678), fused with N-terminal His tag, was expressed in E. coli.
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Species : Human
Source : E.coli
Tag : His
Protein Length : 576-678 a.a.
Form : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose.
Bio-activity : Signal transducer and activator of transcription 3 (STAT3) is a member of the STAT protein family. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. STAT3 is phosphorylated by receptor-associated Janus kinases (JAK), form homo- or heterodimers, and translocate to the cell nucleus where they act as transcription activators. Besides, Epidermal Growth Factor Receptor 2 (EGFR2) has been identified as an interactor of STAT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human STAT3 and recombinant human EGFR2. Briefly, STAT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to EGFR2-coated microtiter wells and incubated for 2h at 37°C . Wells were washed with PBST and incubated for 1h with anti-STAT3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of STAT3 and EGFR2wasshown in Figure, and this effect was in a dose dependent manner.
Molecular Mass : 12.9kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method.
Purity : >95%
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name STAT3 signal transducer and activator of transcription 3 (acute-phase response factor) [ Homo sapiens ]
Official Symbol STAT3
Synonyms STAT3; signal transducer and activator of transcription 3 (acute-phase response factor); signal transducer and activator of transcription 3; APRF; DNA-binding protein APRF; acute-phase response factor; HIES; FLJ20882; MGC16063;
Gene ID 6774
mRNA Refseq NM_003150
Protein Refseq NP_003141
MIM 102582
UniProt ID P40763

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