Recombinant Human STAT1, His-tagged

• Cat.No.: STAT1-72H
• Product Overview: STAT1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 732 amino acids (1-712 a.a.) and having a molecular mass of 85.2 kDa. The STAT1 is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: STAT1 is a member of the Signal Transducers and Activators of Transcriptionfamily of transcription factors. STAT1 is involved in upregulating genes due to a signal by either type Ior type IIinterferons. In response to IFN-γ stimulation, STAT1 forms homodimers or heterodimers with STAT3that bind to the GAS (Interferon-Gamma Activated Sequence) promoter element; in response to either IFN-α or IFN-β stimulation, STAT1 forms a heterodimer with STAT2that can bind the ISRE (Interferon Stimulated Response Element) promoter element. In either case, binding of the promoter element leads to an increased expression of ISG (Interferon Stimulated Genes).
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSQWYELQQL DSKFLEQVHQ LYDDSFPMEI RQYLAQWLEK QDWEHAANDV SFATIRFHDL LSQLDDQYSR FSLENNFLLQ HNIRKSKRNL QDNFQEDPIQ MSMIIYSCLK EERKILENAQ RFNQAQSGNI QSTVMLDKQK ELDSKVRNVK DKVMCIEHEI KSLEDLQDEY DFKCKTLQNR EHETNGVAKS DQKQEQLLLK KMYLMLDNKR KEVVHKIIEL LNVTELTQNA LINDELVEWK RRQQSACIGG PPNACLDQLQ NWFTIVAESL QQVRQQLKKL EELEQKYTYE HDPITKNKQV LWDRTFSLFQ QLIQSSFVVE RQPCMPTHPQ RPLVLKTGVQ FTVKLRLLVK LQELNYNLKV KVLFDKDVNE RNTVKGFRKF NILGTHTKVM NMEESTNGSL AAEFRHLQLK EQKNAGTRTN EGPLIVTEEL HSLSFETQLC QPGLVIDLET TSLPVVVISN VSQLPSGWAS ILWYNMLVAE PRNLSFFLTP PCARWAQLSE VLSWQFSSVT KRGLNVDQLN MLGEKLLGPN ASPDGLIPWT RFCKENINDK NFPFWLWIES ILELIKKHLL PLWNDGCIMG FISKERERAL LKDQQPGTFL LRFSESSREGAITFTWVERS QNGGEPDFHA VEPYTKKELS AVTFPDIIRN YKVMAAENIP ENPLKYLYPN IDKDHAFGKY YSRPKEAPEP MELDGPKGTG YIKTELISVS EV.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Formulation: STAT1 0.5mg/ml protein solution contains 20 mM Tris-HCl buffer pH-8, 0.1M Nacl, 1mM DTT, and 10% glycerol.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: STAT1 signal transducer and activator of transcription 1, 91kDa [ Homo sapiens ]
• Synonyms: STAT1; signal transducer and activator of transcription 1, 91kDa; transcription factor ISGF-3 components p91/p84; Signal transducer and activator of transcription 1-alpha/beta; ISGF-3; STAT91; DKFZp686B04100; signal transducer and activator of transcription 1; OTTHUMP00000205845; signal transducer and activator of transcription-1; OTTHUMP00000165047; Transcription factor ISGF-3 components p91/p84
• Gene ID: 6772
• mRNA Refseq: NM_007315
• Protein Refseq: NP_009330
• MIM: 600555
• UniProt ID: P42224
• Chromosome Location: 2q32.2-q32.3
• Pathway: Chemokine signaling pathway; Jak-STAT signaling pathway; Pancreatic cancer; Pathways in cancer; Toll-like receptor signaling pathway; Signaling by PDGF
• Function: calcium ion binding; hematopoietin/interferon-class (D200-domain) cytokine receptor signal transducer activity; protein binding; sequence-specific DNA binding; signal transducer activity; transcription factor activity