Recombinant Human SRSF1 protein, His-tagged

• Cat.No.: SRSF1-5264H
• Product Overview: Recombinant Human SRSF1(1-248aa) fused with His tag at C-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-248 a.a.
• Description: SRSF1, also known as Serine/arginine-rich splicing factor 1, is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. This protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. In addition to being involved in the splicing process, it also mediates post-splicing activities, such as mRNA nuclear export and translation.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 100mM KCl, 1mM DTT, 0.2mM EDTA, 40% Glycerol
• Molecular Mass: 28.5kDa (254aa) 28-40KDa (SDS-PAGE under reducing conditions.)
• AA Sequence: MSGGGVIRGP AGNNDCRIYV GNLPPDIRTK DIEDVFYKYG AIRDIDLKNR RGGPPFAFVE FEDPRDAEDA VYGRDGYDYD GYRLRVEFPRSGRGTGRGGG GGGGGGAPRG RYGPPSRRSE NRVVVSGLPP SGSWQDLKDH MREAGDVCYA DVYRDGTGVV EFVRKEDMTY AVRKLDNTKFRSHEGETAYI RVKVDGPRSP SYGRSRSRSR SRSRSRSRSN SRSRSYSPRR SRGSPRYSPR HSRSRSRTHH HHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford Assay)
• Publications:
  SRPK1/2 and PP1α exert opposite functions by modulating SRSF1-guided MKNK2 alternative splicing in colon adenocarcinoma (2021)

Gene Information

• Gene Name: SRSF1 serine/arginine-rich splicing factor 1 [ Homo sapiens ]
• Official Symbol: SRSF1
• Synonyms: SRSF1; serine/arginine-rich splicing factor 1; SFRS1, splicing factor, arginine/serine rich 1; alternate splicing factor; ASF; MGC5228; pre mRNA splicing factor SF2; P33 subunit; SF2; SF2p33; splicing factor 2; SR splicing factor 1; SRp30a; ASF-1; alternative-splicing factor 1; splicing factor, arginine/serine-rich 1; pre-mRNA-splicing factor SF2, P33 subunit; SFRS1; FLJ53078
• Gene ID: 6426
• mRNA Refseq: NM_006924
• Protein Refseq: NP_008855
• MIM: 600812
• UniProt ID: Q07955
• Chromosome Location: 17q22
• Pathway: Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem; Gene Expression, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; RNA Polymerase II Transcription, organism-specific biosystem; RNA Polymerase II Transcription Termination, organism-specific biosystem
• Function: RNA binding; RS domain binding; nucleotide binding; protein binding