Recombinant Human sprouty-related, EVH1 domain containing 1, His-tagged

• Cat.No.: SPRED1-630H
• Product Overview: SPRED1, 1-444aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-444 a.a.
• Description: SPRED1 is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. This protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). Recombinant human SPRED1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl (pH 8.0) containing 10% glycerol
• Molecular Mass: 52.9 kDa (467aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSEETAT SDNDNSYARV RAVVMTRDDS SGGWLPLGGS GLSSVTVFKV PHQEENGCAD FFIRGERLRD KMVVLECMLK KDLIYNKVTP TFHHWKIDDK KFGLTFQSPA DARAFDRGIR RAIEDISQGC PESKNEAEGA DDLQANEEDS SSSLVKDHLF QQETVVTSEP YRSSNIRPSP FEDLNARRVY MQSQANQITF GQPGLDIQSR SMEYVQRQIS KECGSLKSQN RVPLKSIRHV SFQDEDEIVR INPRDILIRR YADYRHPDMW KNDLERDDAD SSIQFSKPDS KKSDYLYSCG DETKLSSPKD SVVFKTQPSS LKIKKSKRRK EDGERSRCVY CQERFNHEEN VRGKCQDAPD PIKRCIYQVS CMLCAESMLY HCMSDSEGDF SDPCSCDTSD DKFCLRWLAL VALSFIVPCM CCYVPLRMCH RCGEACGCCG GKHKAAG
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SPRED1 sprouty-related, EVH1 domain containing 1 [ Homo sapiens ]
• Official Symbol: SPRED1
• Synonyms: SPRED1; sprouty-related, EVH1 domain containing 1; sprouty-related, EVH1 domain-containing protein 1; FLJ33903; hSpred1; spred-1; suppressor of Ras/MAPK activation; EVH1/Sprouty domain containing protein; NFLS
• Gene ID: 161742
• mRNA Refseq: NM_152594
• Protein Refseq: NP_689807
• MIM: 609291
• UniProt ID: Q7Z699
• Chromosome Location: 15q14
• Pathway: Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; Kit Receptor Signaling Pathway, organism-specific biosystem; Signaling events mediated by Stem cell factor receptor (c-Kit), organism-specific biosystem
• Function: phosphatase binding; protein binding; stem cell factor receptor binding