Recombinant Human SPHK1 protein, His&GST-tagged

• Cat.No.: SPHK1-0259H
• Product Overview: Recombinant Human SPHK1 protein(Q9NYA1-1)(Met1-Leu384), fused with His and GST tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His&GST
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 8.0.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The secreted recombinant human SPHK1 consists of 621 amino acids and predicts a molecular mass of 70.3 KDa. The apparent molecular mass of the protein is approximately 64 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Proteinlength: Met1-Leu384
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 1-384 a.a.

Gene Information

• Gene Name: SPHK1 sphingosine kinase 1 [ Homo sapiens ]
• Official Symbol: SPHK1
• Synonyms: SPHK1; sphingosine kinase 1; SPHK; SK 1; SPK 1
• Gene ID: 8877
• mRNA Refseq: NM_001142601
• Protein Refseq: NP_001136073
• MIM: 603730
• UniProt ID: Q9NYA1