Recombinant Human SPANXB1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: SPANXB1-2497H
• Product Overview: SPANXB1 MS Standard C13 and N15-labeled recombinant protein (NP_115850) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular family member contains an additional 18 nucleotides in its coding region compared to the other family members in the same gene cluster. This family member is also subject to gene copy number variation. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis.
• Molecular Mass: 11.8 kDa
• AA Sequence: MGQQSSVRRLKRSVPCESNEANEANEANKTMPETPTGDSDPQPAPKKMKTSESSTILVVRYRRNVKRTSPEELVNDHARENRINPDQMEEEEFIEITTERPKKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: SPANXB1 SPANX family member B1 [ Homo sapiens (human) ]
• Official Symbol: SPANXB1
• Synonyms: SPANXB1; SPANX family, member B1; cancer/testis antigen family 11; member 2; CT11.2; SPANX; SPANXB; SPANX-B
• Gene ID: 728695
• mRNA Refseq: NM_032461
• Protein Refseq: NP_115850
• MIM: 300669
• UniProt ID: Q9NS25