Recombinant Human SPAG1 protein, His & T7-tagged

• Cat.No.: SPAG1-8123H
• Product Overview: Recombinant Human SPAG1 aa. (Met1~Lys365 (Accession # Q07617)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 45.6kDa
• Protein length: Met1~Lys365
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.5.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: SPAG1 sperm associated antigen 1 [ Homo sapiens (human) ]
• Official Symbol: SPAG1
• Synonyms: SP75; TPIS; CT140; CILD28; HSD-3.8; HEL-S-268; TPR-containing protein involved in spermatogenesis; epididymis secretory protein Li 268; infertility-related sperm protein Spag-1; tetratricopeptide repeat-containing protein; sperm-associated antigen 1
• Gene ID: 6674
• mRNA Refseq: NM_003114.4
• Protein Refseq: NP_003105.2
• UniProt ID: Q07617