Recombinant Human SPAG1 Protein (Met1-Lys365), N-His tagged

• Cat.No.: SPAG1-5474H
• Product Overview: Recombinant human SPAG1 (Met1-Lys365) fused with the N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1-Lys365
• Description: The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein.
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 44.23 kDa
• Purity: > 90 % as determined by SDS-PAGE
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: 0.01M PBS, pH 7.4, 0.02 % NLS
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: SPAG1 sperm associated antigen 1 [ Homo sapiens (human) ]
• Official Symbol: SPAG1
• Synonyms: SPAG1; sperm associated antigen 1; SP75; TPIS; CT140; CILD28; DNAAF13; HSD-3.8; HEL-S-268; sperm-associated antigen 1; TPR-containing protein involved in spermatogenesis; dynein axonemal assembly factor 13; epididymis secretory protein Li 268; infertility-related sperm protein Spag-1; tetratricopeptide repeat-containing protein
• Gene ID: 6674
• mRNA Refseq: NM_001374321
• Protein Refseq: NP_001361250
• MIM: 603395
• UniProt ID: Q07617