Recombinant Human solute carrier family 16 member 3 Protein, His&Flag&StrepII tagged

• Cat.No.: SLC16A3-030H
• Product Overview: Recombinant human SLC16A3/MCT4 protein (O15427) ((Asn 406-Val 465)-G4S-(Asn 406-Val 465)) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Description: MCT4 (SLC16A3) is the third member of the monocarboxylate transporter (MCT) family and is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate, and ketone bodies. MCT1 and MCT4 transport pH-related monocarboxylates, such as lactate and pyruvate. MCT11 may also be a proton-coupled monocarboxylate transporter. Monocarboxylate transporter 4 (MCT4), encoded by the SLC16A3 gene, is responsible for exporting lactic acid into the extracellular microenvironment, and an acidic microenvironment promotes cytokine production and remodels chronic inflammation, providing a link from glycolysis to inflammatory bowel disease (IBD). Overexpressed Solute Carrier Family 16 Member 3 (SLC16A3) plays a critical role in hypoxic cancer cell growth and proliferation, by expelling glycolysis-derived lactate across the plasma membrane.
• Source: E. coli
• Species: Human
• Tag: N-Twin strep and C-His&Flag
• Protein length: 406-465aa
• Molecular Mass: 20.5 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: SLC16A3 solute carrier family 16 member 3 [ Homo sapiens (human) ]
• Official Symbol: SLC16A3
• Synonyms: MCT3; MCT4; MCT 3; MCT 4; MCT-3; MCT-4
• Gene ID: 9123
• mRNA Refseq: NM_004207.3
• Protein Refseq: NP_004198.1
• MIM: 603877
• UniProt ID: O15427