Recombinant Human SNRPF, His-tagged

• Cat.No.: SNRPF-3366H
• Product Overview: Recombinant human SNRPF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 11.8 kDa (106aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Small nuclear ribonucleoprotein polypeptide F, also known SNRPF, belongs to the snRNP Sm proteins family. There are at least seven isoforms, E, F, G, D1, D2, D3 and B/B. This class of common proteins plays an essential role in the biogenesis of the snRNPs. In addition, these proteins represent the major targets for the so-called anti-Sm auto-antibodies which are diagnostic for systemic lupus erythematosus (SLE). SNRPF aid in the cytoplasmic construction of the UsnRNPs by binding to aconserved Sm site on UsnRNA and forming a stable snRNP core complex.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 1mM EDTA.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSLPLNPKPF LNGLTGKPVM VKLKWGMEYKG Y LVSVDGYM NMQLANTEEYIDGALSGHLG EVLIRCNNVLYIRGVEEEEEDGE M R E .
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Spliceosome; Gene Expression; Metabolism of RNA; Transcription; mRNA Processing

Gene Information

• Gene Name: SNRPF small nuclear ribonucleoprotein polypeptide F [ Homo sapiens ]
• Synonyms: small nuclear ribonucleoprotein polypeptide F;SMF; Sm-F; SNRPF; snRNP-F; sm protein F; Sm protein F; small nuclear ribonucleoprotein F; PBSCF
• Gene ID: 6636
• mRNA Refseq: NM_003095
• Protein Refseq: NP_003086
• MIM: 603541
• UniProt ID: P62306
• Chromosome Location: 12q23.1
• Function: protein binding; RNA binding