Recombinant Human SNAPC3 293 Cell Lysate

• Cat.No.: SNAPC3-1637HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for small nuclear RNA activating complex, polypeptide 3, 50kDa (SNAPC3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SNAPC3 small nuclear RNA activating complex, polypeptide 3, 50kDa [ Homo sapiens ]
• Official Symbol: SNAPC3
• Synonyms: SNAP50; PTFbeta; snRNA-activating protein complex subunit 3; SNAPc subunit 3; PTF subunit beta; SNAPc 50 kDa subunit; PSE-binding factor subunit beta; snRNA-activating protein complex 50 kDa subunit; small nuclear RNA-activating complex polypeptide 3; small nuclear RNA activating complex, polypeptide 3, 50kD; proximal sequence element-binding transcription factor subunit beta
• Gene ID: 6619
• mRNA Refseq: NM_001039697
• Protein Refseq: NP_001034786
• MIM: 602348
• UniProt ID: Q92966
• Chromosome Location: 9p22.3
• Pathway: Gene Expression, organism-specific biosystem; RNA Polymerase III Abortive And Retractive Initiation, organism-specific biosystem
• Function: DNA binding