Recombinant Human SMUG1, His-tagged

• Cat.No.: SMUG1-7066H
• Product Overview: Recombinant human SMUG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Single-strand-selective monofunctional uracil-DNA glycosylase, also known as SMUG1, is a enzyme responsible for recognizing base lesions in the genome and initiating base excision DNA repair. This protein acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl,30% glycerol, 1mM DTT
• Molecular Mass: 32.3 kDa (293aa)
• Protein length: 1-270aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPQAFLL GSIHEPAGAL MEPQPCPGSL AESFLEEELR LNAELSQLQF SEPVGIIYNP VEYAWEPHRN YVTRYCQGPK EVLFLGMNPG PFGMAQTGVP FGEVSMVRDW LGIVGPVLTP PQEHPKRPVL GLECPQSEVS GARFWGFFRN LCGQPEVFFH HCFVHNLCPL LFLAPSGRNL TPAELPAKQR EQLLGICDAA LCRQVQLLGV RLVVGVGRLA EQRARRALAG LMPEVQVEGL LHPSPRNPQA NKGWEAVAKE RLNELGLLPL LLK
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: SMUG1 single-strand-selective monofunctional uracil-DNA glycosylase 1 [ Homo sapiens ]
• Official Symbol: SMUG1
• Synonyms: SMUG1; single-strand-selective monofunctional uracil-DNA glycosylase 1; single-strand selective monofunctional uracil DNA glycosylase; FDG; HMUDG; UNG3; MGC104370
• Gene ID: 23583
• mRNA Refseq: NM_001243787
• Protein Refseq: NP_001230716
• MIM: 607753
• UniProt ID: Q53HV7
• Chromosome Location: 12q13.11-q13.3
• Pathway: Base Excision Repair, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Base-Excision Repair, AP Site Formation, organism-specific biosystem; Base-free sugar-phosphate removal via the single-nucleotide replacement pathway, organism-specific biosystem; Cleavage of the damaged pyrimidine, organism-specific biosystem; DNA Repair, organism-specific biosystem
• Function: DNA N-glycosylase activity; DNA binding; hydrolase activity, acting on glycosyl bonds; protein binding; single-strand selective uracil DNA N-glycosylase activity; uracil DNA N-glycosylase activity