Recombinant Human SMPDL3B Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: SMPDL3B-3075H
• Product Overview: SMPDL3B MS Standard C13 and N15-labeled recombinant protein (NP_001009568) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling. Has in vitro phosphodiesterase activity, but the physiological substrate is unknown. Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro).
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 41.7 kDa
• AA Sequence: MRLLAWLIFLANWGGARAEPGKFWHIADLHLDPDYKVSKDPFQVCPSAGSQPVPDAGPWGDYLCDSPWALINSSIYAMKEIEPEPDFILWTGDDTPHVPDEKLGEAAVLEIVERLTKLIREVFPDTKVYAALGNHDFHPKNQFPAGSNNIYNQIAELWKPWLSNESIALFKKGAFYCEKLPGPSGAGRIVVLNTNLYYTSNALTADMADPGQQFQWLEDVLTDASKAGDMVYIVGHVPPGFFEKTQNKAWFREGFNEKYLKVVRKHHRVIAGQFFGHHHTDSFRMLYDDAGVPISAMFITPGVTPWKTTLPGVVNGANNPAIRVFEYDRATLSLKVRSPAEARGGGWEGLKCITTFPHSQLIHLPLTTEPQEGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: SMPDL3B sphingomyelin phosphodiesterase acid like 3B [ Homo sapiens (human) ]
• Official Symbol: SMPDL3B
• Synonyms: SMPDL3B; sphingomyelin phosphodiesterase, acid-like 3B; acid sphingomyelinase-like phosphodiesterase 3b; ASML3B; ASM-like phosphodiesterase 3b
• Gene ID: 27293
• mRNA Refseq: NM_001009568
• Protein Refseq: NP_001009568
• MIM: 617737
• UniProt ID: Q92485