Recombinant Human SMAD Family Member 5, GST-tagged

• Cat.No.: SMAD5-1096H
• Product Overview: Recombinant full-length human SMAD5 was expressed in E. coli cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: SMAD5 is a member of the SMAD family and mediates signaling by the transforming growth factor-beta (TGFβ) superfamily and related ligands. SMAD5 plays a critical role in the signaling pathway by which TGFβ inhibits the proliferation of human hematopoietic progenitor cells. SMAD5 is up-regulated in gastric epithelial cells during the infection of the pathogen Helicobacter pylori and it mediates apoptosis of gastric epithelial cells induced by H. pylori infection. In mature human B cells, bone morphogenetic protein 6 (BMP-6) inhibits cell growth and rapidly induces phosphorylation of SMADs 5 and 8.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 82 kDa
• Form: Recombinant protein stored in 50 mM Tris-HCL, pH 7.5, 150 mM NaCl, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
• Purity: > 90 %
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: ALK1 signaling events; ALK2 signaling events; BMP receptor signaling; Id Signaling Pathway; Signaling by BMP; TGF Beta Signaling Pathway; TGF-beta signaling pathway

Gene Information

• Gene Name: SMAD5 SMAD family member 5 [ Homo sapiens ]
• Official Symbol: SMAD5
• Synonyms: SMAD5; SMAD family member 5; DWFC; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; MAD, mothers against decapentaplegic homolog 5 (Drosophila); SMAD, mothers against DPP homolog 5 (Drosophila); Dwfc; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; Smad5; hSmad5; OTTHUMP00000223331; mothers against decapentaplegic, drosophila, homolog of, 5; SMA- and MAD-related protein 5; SMAD, mothers against DPP homolog 5; SMAD 5
• Gene ID: 4090
• mRNA Refseq: NM_005903
• Protein Refseq: NP_005894
• MIM: 603110
• UniProt ID: Q99717
• Chromosome Location: 5q31
• Function: protein binding; receptor signaling protein activity; transcription activator activity; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity