Recombinant Human SMAD Family Member 2, His-tagged

• Cat.No.: SMAD2-392H
• Product Overview: Recombinant SMAD2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 54.4 kDa (487aa).

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-467 a.a.
• Description: SMAD2(Mothers against decapentaplegic homolog 2) belongs to the SMAD family of proteins that mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. Phosphorylated Smad2 is then able to form a complex with Smad4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSSILPFTPP VVKRLLGWKK SAGGSGGAGG GEQNGQEEKW CEKAVKSLVK KLKKTGRLDE LEKAITTQNC NTKCVTIPST CSEIWGLSTP NTIDQWDTTG LYSFSEQTRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH HELKAIENCE YAFNLKKDEV CVNPYHYQRV ETPVLPPVLV PRHTEILTEL PPLDDYTHSI PENTNFPAGI EPQSNYIPET PPPGYISEDG ETSDQQLNQS MDTGSPAELS PTTLSPVNHS LDLQPVTYSE PAFWCSIAYY ELNQRVGETF HASQPSLTVD GFTDPSNSER FCLGLLSNVN RNATVEMTRR HIGRGVRLYY IGGEVFAECL SDSAIFVQSP NCNQRYGWHP ATVCKIPPGC NLKIFNNQEF AALLAQSVNQ GFEAVYQLTR MCTIRMSFVK GWGAEYRRQT VTSTPCWIEL HLNGPLQWLD KVLTQMGSPS VRCSSMS
• Formulation: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 100 mM NaCl
• Purity: > 95% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: SMAD family member 2 [ Homo sapiens ]
• Synonyms: SMAD2; SMAD family member 2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; MGC22139; MGC34440; Sma- and Mad-related protein 2; Mad protein homolog; Mad-related protein 2; mother against DPP homolog 2; SMAD, mothers against DPP homolog 2; MAD, mothers against decapentaplegic homolog 2
• Gene ID: 4087
• mRNA Refseq: NM_001003652
• Protein Refseq: NP_001003652
• MIM: 601366
• UniProt ID: Q15796
• Chromosome Location: 18q21.1
• Pathway: Adherens junction; Cell cycle; Colorectal cancer; Pancreatic cancer; TGF-beta signaling pathway; Wnt signaling pathway; Signaling by TGF beta
• Function: R-SMAD binding; co-SMAD binding; double-stranded DNA binding; promoter binding; transcription activator activity; transcription factor activity; transcription factor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; type I transforming growth factor beta receptor binding; ubiquitin protein ligase binding