Recombinant Human SLC9A3R1 293 Cell Lysate

• Cat.No.: SLC9A3R1-1694HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SLC9A3R1 solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1 [ Homo sapiens ]
• Official Symbol: SLC9A3R1
• Synonyms: SLC9A3R1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1 , solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 , solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; EBP50; NHERF; Na+/H+ exchange regulatory co-factor; regulatory cofactor of Na(+)/H(+) exchanger; ezrin-radixin-moesin binding phosphoprotein-50; ezrin-radixin-moesin-binding phosphoprotein 50; solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; NHERF1; NHERF-1; NPHLOP2
• Gene ID: 9368
• mRNA Refseq: NM_004252
• Protein Refseq: NP_004243
• MIM: 604990
• UniProt ID: O14745
• Chromosome Location: 17q25.1
• Pathway: PDGFR-beta signaling pathway, organism-specific biosystem; Thromboxane A2 receptor signaling, organism-specific biosystem
• Function: PDZ domain binding; beta-2 adrenergic receptor binding; beta-catenin binding; chloride channel regulator activity; dopamine receptor binding; growth factor receptor binding; phosphatase binding; protein binding; protein self-association; receptor binding