Recombinant Human SLC4A4, His-tagged

• Cat.No.: SLC4A4-31418TH
• Product Overview: Recombinant fragment of Human SLC4A4 with a 37 amino acid N terminal His tag; 460 amino acids, predicted MWt 51.5 kDa including tag.

Specification

• Description: This gene encodes a sodium bicarbonate cotransporter (NBC) involved in the regulation of bicarbonate secretion and absorption and intracellular pH. Mutations in this gene are associated with proximal renal tubular acidosis. Multiple transcript variants encoding different isoforms have been found for this gene.
• Protein length: 424 amino acids
• Conjugation: HIS
• Molecular Weight: 51.500kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 i
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: pH: 7.50Constituents:0.32% Tris HCl, 0.01% DTT, 0.002% PMSF, 10% Glycerol
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSTE NVEGKPSNLG ERGRARSSTF LRVVQPMFNH SIFTSAVSPA AERIRFILGEEDDSPAPPQL FTELDELLAV DGQEMEWKET ARWIKFEEKV EQGGERWSKP HVATLSLHSL FELRTCMEKG SIMLDREASS LPQLVEMIVD HQIETGLLKP ELKDKVTYTL LRKHRHQTKK SNLRSLADIG KTVSSASRMF TNPDNGSPAM THRNLTSSSL NDISDKPEKD QLKNKFMKKLPRDAEASNVL VGEVDFLDTP FIAFVRLQQA VMLGALTEVP VPTRFLFILL GPKGKAKSYH EIGRAIATLM SDEVFHDIAY KAKDRHDLIA GIDEFLDEVI VLPPGEWDPA IRIEPPKSLP SSDKRKNMYS GGENVQMNGD TPHDGGHGGG GHGDCEELQR TGRFCGGLIK DIKRKAPFFASDFYDALNIQ
• Sequence Similarities: Belongs to the anion exchanger (TC 2.A.31) family.

Gene Information

• Gene Name: SLC4A4 solute carrier family 4, sodium bicarbonate cotransporter, member 4 [ Homo sapiens ]
• Official Symbol: SLC4A4
• Synonyms: SLC4A4; solute carrier family 4, sodium bicarbonate cotransporter, member 4; SLC4A5; electrogenic sodium bicarbonate cotransporter 1; hhNMC; HNBC1; NBC1; NBC2; pNBC
• Gene ID: 8671
• mRNA Refseq: NM_001098484
• Protein Refseq: NP_001091954
• MIM: 603345
• Uniprot ID: Q9Y6R1
• Chromosome Location: 4q21
• Pathway: Bicarbonate transporters, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Pancreatic secretion, organism-specific biosystem; Pancreatic secretion, conserved biosystem
• Function: anion transmembrane transporter activity; inorganic anion exchanger activity; protein binding; sodium:bicarbonate symporter activity; symporter activity