Recombinant Human SLC2A5 293 Cell Lysate

• Cat.No.: SLC2A5-1740HCL

Specification

• Description: Antigen standard for solute carrier family 2 (facilitated glucose/fructose transporter), member 5 (SLC2A5), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: SLC2A5 solute carrier family 2 (facilitated glucose/fructose transporter), member 5 [ Homo sapiens ]
• Official Symbol: SLC2A5
• Synonyms: SLC2A5; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; GLUT5; solute carrier family 2, facilitated glucose transporter member 5; glucose transporter-like protein 5; glucose transporter type 5, small intestine; GLUT-5
• Gene ID: 6518
• mRNA Refseq: NM_001135585
• Protein Refseq: NP_001129057
• MIM: 138230
• UniProt ID: P22732
• Chromosome Location: 1p36.2
• Pathway: Carbohydrate digestion and absorption, organism-specific biosystem; Carbohydrate digestion and absorption, conserved biosystem; Class II GLUTs, organism-specific biosystem; Facilitative Na+-independent glucose transporters, organism-specific biosystem; Glycolysis and Gluconeogenesis, organism-specific biosystem; Hexose transport, organism-specific biosystem; Metabolism, organism-specific biosystem
• Function: fructose transmembrane transporter activity; glucose transmembrane transporter activity; substrate-specific transmembrane transporter activity