Recombinant Human SLC28A1 293 Cell Lysate

• Cat.No.: SLC28A1-1746HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for solute carrier family 28 (sodium-coupled nucleoside transporter), member 1 (SLC28A1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SLC28A1 solute carrier family 28 (concentrative nucleoside transporter), member 1 [ Homo sapiens ]
• Official Symbol: SLC28A1
• Synonyms: CNT1; HCNT1; sodium/nucleoside cotransporter 1; Na(+)/nucleoside cotransporter 1; solute carrier family 28 member 1; concentrative nucleoside transporter 1; sodium-coupled nucleoside transporter 1; solute carrier family 28 (sodium-coupled nucleoside transporter), member 1
• Gene ID: 9154
• mRNA Refseq: NM_004213
• Protein Refseq: NP_004204
• MIM: 606207
• Chromosome Location: 15q25.3
• Pathway: SLC-mediated transmembrane transport, organism-specific biosystem; Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane, organism-specific biosystem; Transport of vitamins, nucleosides, and related molecules, organism-specific biosystem
• Function: nucleoside binding; nucleoside transmembrane transporter activity; nucleoside:sodium symporter activity