Recombinant Human SIT1 293 Cell Lysate

• Cat.No.: SIT1-1827HCL

Specification

• Description: Antigen standard for signaling threshold regulating transmembrane adaptor 1 (SIT1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: SIT1 signaling threshold regulating transmembrane adaptor 1 [ Homo sapiens ]
• Official Symbol: SIT1
• Synonyms: SIT1; signaling threshold regulating transmembrane adaptor 1; suppression inducing transmembrane adaptor 1; signaling threshold-regulating transmembrane adapter 1; SHP2 interacting transmembrane adaptor; SIT; gp30/40; suppression-inducing transmembrane adapter 1; SHP2-interacting transmembrane adapter protein; SHP2-interacting transmembrane adaptor protein; RP11-331F9.5; MGC125908; MGC125909; MGC125910
• Gene ID: 27240
• mRNA Refseq: NM_014450
• Protein Refseq: NP_055265
• MIM: 604964
• UniProt ID: Q9Y3P8
• Chromosome Location: 9p13-p12
• Pathway: T Cell Receptor Signaling Pathway, organism-specific biosystem
• Function: SH2 domain binding; kinase binding; protein binding