Recombinant Human Sirtuin 6, GST-tagged

• Cat.No.: SIRT6-4883H
• Product Overview: Full-length recombinant human SIRT6 was expressed in E. coli cells using an N-terminal GST tag.

Specification

• Cat. No.: SIRT6-4883H
• Description: SIRT6 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT6 being a member of class IV. Human SIRT6 protein is a NAD (+)-dependent histone H3 lysine-9 deacetylase that modulates telomeric chromatin. SIRT6 associates specifically with telomeres and SIRT6 depletion leads to telomere dysfunction with end-to-end chromosomal fusions and premature cellular senescence.
• Applications: Western Blot
• Molecular Weight: 66 kDa
• Expression System: E. coli
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1mM PMSF, 25 % glycerol.
• Purity: > 85 %
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Signaling events mediated by HDAC Class I

Gene Information

• Gene Name: SIRT6 sirtuin 6 [ Homo sapiens ]
• Official Symbol: SIRT6
• Synonyms: SIRT6; sirtuin 6; SIR2L6; NAD-dependent deacetylase sirtuin-6; sirtuin type 6; SIR2-like protein 6; sir2-related protein type 6; EC 2.4.2.31; sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
• Gene ID: 51548
• mRNA Refseq: NM_016539
• Protein Refseq: NP_057623
• MIM: 606211
• UniProt ID: Q8N6T7
• Chromosome Location: 19p13.3
• Function: NAD binding; NAD (P)+-protein-arginine ADP-ribosyltransferase activity; NAD+ ADP-ribosyltransferase activity; hydrolase activity; metal ion binding; protein binding; transferase activity, transferring glycosyl groups; zinc ion binding; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific)

Reviews

07/21/2022

This protein has high yield and good economic benefits.

05/25/2022

Laboratory staff feedback that the protein is easy to operate, can meet the needs of the experiment, and has a good cost performance.

12/13/2021

SIRT6 is highly expressed and can efficiently synthesize a large number of target proteins in host cells.

Q&As

How is SIRT6 involved in DNA repair?

SIRT6 can participate in the DNA repair process by influencing the activity of DNA repair enzymes through deacetylation modification.

What is the nutritional significance of SIRT6?

This proteinexpression of genes related to fat metabolism through deacetylation modifications.

Does SIRT6 have therapeutic potential?

Yes, SIRT6 has therapeutic potential. In tumor therapy, drug activation or gene therapy targeting SIRT6 may become a new avenue for treating tumors. At the same time, activators for SIRT6 are also being developed.

How does SIRT6 affect nutrient metabolism and energy balance?

SIRT6 regulates the expression of specific genes through deacetylation, which in turn affects nutrient metabolism and energy balance. For example, in adipocytes, activation of SIRT6 may lead to abnormal expression of genes related to adipopocyte, which in turn affects processes such as differentiation, proliferation, and apoptosis of adipocytes.

What is the effect of loss of SIRT6 on cells?

Deletion of SIRT6 may lead to cell growth arrest, increased apoptosis, and genomic instability.

How do the expression levels of SIRT6 differ in different tissues and cells?

There are differences in the expression levels of SIRT6 in different tissues and cells. For example, in tissues and cells such as the reproductive system, nervous system, and immune system, there may be varying degrees of variation in the expression levels of SIRT6.