Recombinant Human Sirtuin 1, His-tagged

Cat. No. :	SIRT1-4873H
Description :	SIRT1 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT1 being a member of class I. SIRT1 is a stress-response and chromatin-silencing factor. It is an NAD (+)-dependent histone deacetylase involved in various nuclear events such as transcription, DNA replication, and DNA repair. SIRT1 protein binds and deacetylates the p53 protein.
Applications :	SIRT Assay
Molecular Weight :	84 kDa
Expression System :	Sf9 insect cells using baculovirus
Species :	Human
Form :	Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
Specific Activity :	2100 RLU/min/mg
Purity :	> 70 %
Concentration :	0.1 ug/ul
Sequences :	193-end
Storage :	Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Pathways :	E2F transcription factor network; Energy Metabolism; FoxO family signaling; HIF-2-alpha transcription factor network; Regulation of Androgen receptor activity; Regulation of retinoblastoma protein; Signaling events mediated by HDAC Class I; Signaling events mediated by HDAC Class III
Protein length :	193-end a.a.

Gene Name	SIRT1 sirtuin 1 [ Homo sapiens ]
Official Symbol	SIRT1
Synonyms	SIRT1; sirtuin 1; SIR2L1; NAD-dependent deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; OTTHUMP00000019691; OTTHUMP00000198112; SIR2-like protein 1; EC 3.5.1.-; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; NAD-dependent deacetylase sirtuin-1
Gene ID	23411
mRNA Refseq	NM_001142498
Protein Refseq	NP_001135970
MIM	604479
UniProt ID	Q96EB6
Chromosome Location	10q21
Function	NAD binding; NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity; deacetylase activity; histone binding; hydrolase activity; identical protein binding; metal ion binding; p53 binding; protein C-terminus binding; protein domain specific binding; transcription corepressor activity; zinc ion binding; HLH domain binding