Recombinant Human SIRT3 protein, His-tagged

• Cat.No.: SIRT3-10H
• Product Overview: Recombinant Human SIRT3 protein (Gln126-Lys399) was fused to His-tag at the N-terminus and expressed in E.coli.

Specification

• Description: SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. SIRT3 has far-reaching effects on nuclear gene expression, cancer, cardiovascular disease, neuroprotection, aging, and metabolic control.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder. 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: Predicted band size: 31.7 kDa; Accurate Molecular Mass: 32 kDa.
• Protein length: Gln126-Lys399
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method)
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SIRT3
• Official Symbol: SIRT3
• Synonyms: SIR2L3
• Gene ID: 23410
• mRNA Refseq: NM_012239.6
• Protein Refseq: NP_036371.1
• MIM: 604481
• UniProt ID: Q9NTG7