Active Recombinant Human SIRT3, His-tagged

• Cat.No.: SIRT3-345H
• Product Overview: Recombinant Human SIRT3, fused with N-terminal His-tagged, was expressed in E. coli.

Specification

• Description: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol
• Bio-activity: batch specific nmoles/min/mg; one unit is defined as the amount of enzyme required to produce 1 nmole of 7-amino-4-methylcoumarin per minute at 25°C in 50 mM Tris-HCl, pH 8.0, containing 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 125 µM p53 amino acids 317-320 (Gln-Pro-Lys-Lys(ε-acetyl)-AMC), and 6 mM NAD+
• Molecular Mass: 37 kDa
• Purity: ≥90%
• Storage: -80°C

Gene Information

• Gene Name: SIRT3 sirtuin 3 [ Homo sapiens (human) ]
• Official Symbol: SIRT3
• Synonyms: SIRT3; sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae); SIR2L3; NAD-dependent deacetylase sirtuin-3, mitochondrial; EC 3.5.1.-; SIR2-like protein 3; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase 2; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3; sirtuin 3; sirtuin type 3
• Gene ID: 23410
• mRNA Refseq: NM_001017524
• Protein Refseq: NP_001017524
• MIM: 604481
• UniProt ID: Q9NTG7
• Chromosome Location: 11p15.5
• Pathway: Energy Metabolism; Mitochondrial biogenesis; Organelle biogenesis and maintenance
• Function: NOT NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity (H3-K14 specific); protein binding