Recombinant Human SIRT2, His-tagged

• Cat.No.: SIRT2-30567TH
• Product Overview: Recombinant fragment, corresponding to amino acids 50-356 of Human SIRT2, with C-terminal His tag, 35.5 kDa.

Specification

• Description: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene.
• Conjugation: HIS
• Source: E. coli
• Tissue specificity: Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulatio
• Biological activity: Specific Activity: 1.4 U/μg. One U =1 pmol/min.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Belongs to the sirtuin family.Contains 1 deacetylase sirtuin-type domain.
• Protein length: 50-356

Gene Information

• Gene Name: SIRT2 sirtuin 2 [ Homo sapiens ]
• Official Symbol: SIRT2
• Synonyms: SIRT2; sirtuin 2; SIR2L, sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae) , sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 2; NAD-dependent deacetylase sirtuin-2
• Gene ID: 22933
• mRNA Refseq: NM_001193286
• Protein Refseq: NP_001180215
• MIM: 604480
• Uniprot ID: Q8IXJ6
• Chromosome Location: 19q13
• Pathway: Signaling events mediated by HDAC Class I, organism-specific biosystem; Signaling events mediated by HDAC Class III, organism-specific biosystem
• Function: NOT NAD+ ADP-ribosyltransferase activity; NAD+ binding; NAD-dependent histone deacetylase activity; histone acetyltransferase binding; histone deacetylase binding

Reviews

04/15/2021

Efficient and knowledgeable customer service—made our purchase straightforward.

11/02/2020

This recombined protein is our go-to for dependable and accurate results.

08/30/2018

Punctual delivery is crucial, and this product arrived right on time.

Q&As

How does SIRT2 participate in the regulation of cellular stress responses?

SIRT2 is involved in stress response pathways, deacetylating and modulating the activity of stress-responsive proteins, contributing to cellular adaptation to various stress conditions.

How does SIRT2 contribute to the regulation of cellular metabolism?

SIRT2 influences cellular metabolism by deacetylating enzymes involved in glycolysis, fatty acid metabolism, and mitochondrial function, impacting overall energy homeostasis.

Explain the molecular mechanisms underlying SIRT2-mediated deacetylation of target proteins.

SIRT2 uses NAD+ as a cofactor to remove acetyl groups from lysine residues, a process that links cellular metabolic state to protein acetylation and deacetylation.

Discuss the role of SIRT2 in the regulation of cell cycle progression.

SIRT2 influences cell cycle progression by deacetylating key regulators, such as cyclin-dependent kinases (CDKs) and checkpoint proteins, impacting cell cycle checkpoints and progression.

Discuss the role of SIRT2 in neurodegenerative diseases and its potential as a therapeutic target.

SIRT2 has been implicated in neurodegenerative diseases, and modulating its activity is explored as a potential therapeutic strategy for conditions such as Parkinson's disease.