Recombinant Human SIRPB2 protein, Fc-tagged

• Cat.No.: SIRPB2-349H
• Product Overview: Recombinant Human SIRPB2 protein (Glu29-Ser364), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 336
• Description: Signal-regulatory protein gamma (SIRPG or SIRP-gamma) is also known as CD172 antigen-like family member B (CD172g), Signal-regulatory protein beta-2 (SIRPB2), is a single-pass type I membrane protein. SIRPG is expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. SIRPG interacts with CD47. On binding with CD47, CD172g mediates cell-cell adhesion.
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 63.7 kDa. The protein migrates as 68-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: SIRPB2
• Official Symbol: SIRPB2
• Synonyms: PTPN1L; PTPNS1L3; dJ776F14.2
• Gene ID: 284759
• mRNA Refseq: NM_001122962.2
• Protein Refseq: NP_001116434.1
• MIM: 605466
• UniProt ID: Q9P1W8