Recombinant Human SIGLEC5 Protein, C-His-tagged

• Cat.No.: SIGLEC5-153H
• Product Overview: Recombinant Human SIGLEC5 Protein with C-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 17-441 a.a.
• Description: Two families of mammalian lectin-like adhesion molecules bind glycoconjugate ligands in a sialic acid-dependent manner: the selectins and the sialoadhesins. The sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members recognizing sialylated ligands. Siglec-5 binds equally to α2,3-linked and α2,6-linked sialic acid. There exist four isoforms of hSiglec-5 possessing three (hSiglec-5-3L and -3C) or four (hSiglec-5-4L and -4S) extracellular domains linked to long (hSiglec-5-3L and -4L) or short (hSiglec-5-4S) cytoplasmic tails or existing as a soluble isoform (hSiglec-5-3C). Siglec-5 is expressed by monocytes and neutrophils, but is absent from leukemic cell lines representing early stages of myelomonocytic differentiation. Siglec-5 may play a role in the diagnosis and monitoring of acute myeloid leukemia (AML).
• Molecular Mass: ~47 kDa
• AA Sequence: EKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEIPYYAEVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRVERGRDVKYSYQQNKLNLEVTALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSWTGNALSPLDPETTRSSELTLTPRPEDHGTNLTCQMKRQGAQVTTERTVQLNVSYAPQTITIFRNGIALEILQNTSYLPVLEGQALRLLCDAPSNPPAHLSWFQGSPALNATPISNTGILELRRVRSAEEGGFTCRAQHPLGFLQIFLNLSVYSLPQLLGPSCSWEAEGLHCRCSFRARPAPSLCWRLEEKPLEGNSSQGSFKVNSSSAGPWANSSLILHGGLSSDLKVSCKAWNIYGSQSGSVLLLQGRSNLGTGVVPAAL
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Concentration: ≥0.5 mg/mL
• Storage Buffer: PBS, 4M Urea, pH7.4

Gene Information

• Gene Name: SIGLEC5 sialic acid binding Ig-like lectin 5 [ Homo sapiens (human) ]
• Official Symbol: SIGLEC5
• Synonyms: SIGLEC5; sialic acid binding Ig-like lectin 5; CD33L2; sialic acid-binding Ig-like lectin 5; CD170; OB BP2; SIGLEC 5; CD33 antigen-like 2; OB-binding protein 2; obesity-binding protein 2; sialic acid-binding immunoglobulin-like lectin 5; OBBP2; OB-BP2; SIGLEC-5
• Gene ID: 8778
• mRNA Refseq: NM_003830
• Protein Refseq: NP_003821
• MIM: 604200
• UniProt ID: O15389