Recombinant Human SIGLEC12 protein, His-tagged

• Cat.No.: SIGLEC12-6712H
• Product Overview: Recombinant Human SIGLEC12 aa. (Lys22~Gly218 (Accession # Q96PQ1)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 24kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Lys22~Gly218
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.8.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SIGLEC12 sialic acid binding Ig like lectin 12 (gene/pseudogene) [ Homo sapiens (human) ]
• Official Symbol: SIGLEC12
• Synonyms: S2V; SLG; SIGLECL1; Siglec-XII; SIGLEC-like 1; sialic acid binding Ig-like lectin 12; sialic acid-binding Ig-like lectin-like 1; sialic acid-binding Ig-like lectin 12
• Gene ID: 89858
• mRNA Refseq: NM_033329.2
• Protein Refseq: NP_201586.1
• UniProt ID: Q96PQ1