Recombinant Human SGK1 Protein, His-tagged

• Cat.No.: SGK1-22H
• Product Overview: Recombinant Human SGK1 Protein is produced by E. coli expression system. This protein is fused with a His tag.

Specification

• Description: SGK1, also known as serum-and glucocorticoid-regulated kinase, is a serine/threonine protein kinase and a member of the AGC subfamily, which includes protein kinases A, G, and C. This protein plays an important role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. It is activated in vitroby 3-phosphoinositide-dependent protein kinase-1 (PDK-1) and in vivoin response to signals that activate phosphatidylinositol (PI) 3-kinase. Recombinant human SGK1 protein was expressed with N-terminal His-tag in High-Five cells using baculovirus expression system and purified by using conventional chromatography techniques.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.2M NaCl,2mM DTT,0.1mM PMSF.
• Molecular Mass: 44.5 kDa
• Protein length: 60-431 aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MISQPQEPEL MNANPSPPPS PSQQINLGPS SNPHAKPSDF HFLKVIGKGS FGKVLLARHK AEEVFYAVKV LQKKAILKKK EEKHIMSERN VLLKNVKHPFLVGLHFSFQT ADKLYFVLDY INGGELFYHL QRERCFLEPR ARFYAAEIAS ALGYLHSLNI VYRDLKPENI LLDSQGHIVL TDFGLCKENI EHNSTTSTFC GTPEYLAPEV LHKQPYDRTVDWWCLGAVLY EMLYGLPPFY SRNTAEMYDN ILNKPLQLKP NITNSARHLL EGLLQKDRTK RLGAKDDFME IKSHVFFSLI NWDDLINKKI TPPFNPNVSG PNDLRHFDPE FTEEPVPNSI GKSPDSVLVT ASVKEAAEAF LGFSYAPPTD SFL
• Purity: > 90% by SDS - PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 centigrade to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SGK1 serum/glucocorticoid regulated kinase 1 [ Homo sapiens ]
• Official Symbol: SGK1
• Synonyms: SGK1; serum/glucocorticoid regulated kinase 1; serum/glucocorticoid regulated kinase , SGK; serine/threonine-protein kinase Sgk1; SGK
• Gene ID: 6446
• mRNA Refseq: NM_001143676
• Protein Refseq: NP_001137148
• MIM: 602958
• UniProt ID: O00141