Recombinant Human SF1 protein, His & T7-tagged

• Cat.No.: SF1-1707H
• Product Overview: Recombinant Human SF1 aa. (Ala2~Thr274 (Accession # Q15637)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Ala2~Thr274
• Description: This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 9.3

Gene Information

• Gene Name: SF1 splicing factor 1 [ Homo sapiens (human) ]
• Official Symbol: SF1
• Synonyms: SF1; splicing factor 1; BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25; mammalian branch point-binding protein; transcription factor ZFM1; zinc finger gene in MEN1 locus; zinc finger protein 162
• Gene ID: 7536
• mRNA Refseq: NM_001178030.1
• Protein Refseq: NP_001171501.1
• UniProt ID: Q15637