Recombinant Human SETDB1 293 Cell Lysate

• Cat.No.: SETDB1-1923HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for SET domain, bifurcated 1 (SETDB1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SETDB1 SET domain, bifurcated 1 [ Homo sapiens ]
• Official Symbol: SETDB1
• Synonyms: SETDB1; SET domain, bifurcated 1; histone-lysine N-methyltransferase SETDB1; ESET; KG1T; KIAA0067; KMT1E; H3-K9-HMTase 4; lysine N-methyltransferase 1E; histone H3-K9 methyltransferase 4; ERG-associated protein with SET domain; ERG-associated protein with a SET domain, ESET; histone-lysine N-methyltransferase, H3lysine-9 specific 4; H3-K9-HMTase4
• Gene ID: 9869
• mRNA Refseq: NM_001145415
• Protein Refseq: NP_001138887
• MIM: 604396
• UniProt ID: Q15047
• Chromosome Location: 1q21
• Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem; Noncanonical Wnt signaling pathway, organism-specific biosystem
• Function: DNA binding; histone-lysine N-methyltransferase activity; methyltransferase activity; protein binding; transferase activity; zinc ion binding