Active Recombinant Human SETD1A protein, GST-tagged

• Cat.No.: SETD1A-198H
• Product Overview: Recombinant Human SETD1A(1418-end) fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 1418-end
• Description: As a member of the mixed lineage leukemia (MLL) family of histone methyltransferase, it specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 500 nmol/min/mg
• Molecular Mass: 61 kDa
• Purity: >70%
• Applications: Methyltransferase Assay
• Stability: 1 year at -70 centigrade from the date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1μg/μl
• Publications:
  Uhrf1 regulates active transcriptional marks at bivalent domains in pluripotent stem cells through Setd1a (2018)

Gene Information

• Gene Name: SETD1A SET domain containing 1A [ Homo sapiens ]
• Official Symbol: SETD1A
• Synonyms: SETD1A; SET domain containing 1A; histone-lysine N-methyltransferase SETD1A; KIAA0339; KMT2F; Set1; hSET1A; lysine N-methyltransferase 2F; SET domain-containing protein 1A; set1/Ash2 histone methyltransferase complex subunit SET1; Set1A
• Gene ID: 9739
• mRNA Refseq: NM_014712
• Protein Refseq: NP_055527
• MIM: 611052
• UniProt ID: O15047
• Chromosome Location: 16p11.2
• Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem
• Function: RNA binding; histone methyltransferase activity (H3-K4 specific); methyltransferase activity; nucleotide binding; protein binding; transferase activity