Recombinant Human SET domain containing 7, histone lysine methyltransferase Protein, His tagged

• Cat.No.: SETD7-121H
• Product Overview: Recombinant human SETD7/SET7/SET9 protein (Q8WTS6) (Phe 52-Lys 344) with a His tag at the C-terminus was expressed in E. coli.

Specification

• Description: Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS.
• Source: E. coli
• Species: Human
• Tag: C-His
• Protein length: 52-344aa
• Molecular Mass: 34.2 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: SETD7 SET domain containing 7, histone lysine methyltransferase [ Homo sapiens (human) ]
• Official Symbol: SETD7
• Synonyms: SETD7; SET domain containing (lysine methyltransferase) 7; histone-lysine N-methyltransferase SETD7; KIAA1717; KMT7; SET7; SET7/9; Set9; H3-K4-HMTase SETD7; lysine N-methyltransferase 7; SET domain-containing protein 7; histone H3-K4 methyltransferase SETD7; histone H3-lysine 4-specific methyltransferase; SET9; FLJ21193
• Gene ID: 80854
• mRNA Refseq: NM_030648
• Protein Refseq: NP_085151
• MIM: 606594
• UniProt ID: Q8WTS6