Recombinant Human SERPINB5 Protein (375 aa)

• Cat.No.: SERPINB5-338S
• Product Overview: Recombinant human Maspin (rhMaspin) produced in E. coli is a single non-glycosylated polypeptide chain containing 375 amino acids. rhMaspin has a molecular mass of 42.1 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Protein Length: 375
• Description: Maspin is a non-inhibitory member of the serpin superfamily, a group of 40-60 kDa serine protease inhibitors. Maspin was discovered in 1994 and has been identified in prostate, thymus, testis, intestine, tongue, and lung. Recently Maspin received a great deal of attention due to its potential apoptotic role in cancers. Maspin expression predicts a better prognosis for several types of carcinomas including breast, prostate, colon, and oral squamus cell carcinoma. The level of Maspin is also inversely correlated to VEGF-A, an angiogenic factor. Researchers found that Maspin may play its apoptotic role as an endogenous inhibitor of histone deacetylase I (HDAC I). Therefore, Maspin is a potential personalized marker of Non-Small Cell Lung Carcinoma (NSCLC).
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: Data not available.
• Molecular Mass: 42.1 kDa, observed by reducing SDS-PAGE.
• AA Sequence: MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDIPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFPESETKECPFRLNKTDTKPVQMMNMEATFCMGNIDSINCKIIELPFQNKHLSMFILLPKDVEDESTGLEKIEKQLNSESLSQWTNPSTMANAKVKLSIPKFKVEKMIDPKACLENLGLKHIFSEDTSDFSGMSETKGVALSNVIHKVCLEITEDGGDSIEVPGARILQHKDELNADHPFIYIIRHNKTRNIIFFGKFCSP
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% by SDS-PAGE and HPLC analysis.
• Storage: Lyophilized recombinant human Maspin (rhMaspin) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhMaspin remains stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Gene Information

• Gene Name: SERPINB5 serpin peptidase inhibitor, clade B (ovalbumin), member 5 [ Homo sapiens ]
• Official Symbol: SERPINB5
• Synonyms: SERPINB5; serpin peptidase inhibitor, clade B (ovalbumin), member 5; PI5, serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; serpin B5; maspin; protease inhibitor 5 (maspin); PI-5; peptidase inhibitor 5; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; PI5
• Gene ID: 5268
• mRNA Refseq: NM_002639
• Protein Refseq: NP_002630
• MIM: 154790
• UniProt ID: P36952