Recombinant Human Serine Racemase, His-tagged

• Cat.No.: SRR-1575H
• Product Overview: Recombinant human SRR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: SRR, also known as serine racemase, is an enzyme which generates D-serine from L-serine. D-serine acts as a neuronal signaling molecule by activating NMDA receptors in the brain. Mammalian serine racemase is a pyridoxal 5"-phosphate dependent enzyme that catalyzes both the racemization of L-serine to D-serine and also the elimination of water from L-serine, generating pyruvate and ammonia. The enzyme is physiologically stimulated by divalent cations (e.g, magnesium) and is allosterically activated by the magnesium/ATP complex.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing20% glycerol, 0.1M NaCl, 1mM DTT.
• Molecular Weight: 39.1 kDa (364aa), confirmed by MALDI-TOF.
• Purity: >85% by SDS-PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMCAQYC ISFADVEKAH INIRDSIHLT PVLTSSILNQ LTGRNLFFKC ELFQKTGSFK IRGALNAVRS LVPDALERKP KAVVTHSSGN HGQALTYAAK LEGIPAYIVV PQTAPDCKKL AIQAYGASIV YCEPSDESRE NVAKRVTEET EGIMVHPNQE PAVIAGQGTI ALEVLNQVPL VDALVVPVGG GGMLAGIAIT VKALKPSVKV YAAEPSNADD CYQSKLKGKL MPNLYPPETI ADGVKSSIGL NTWPIIRDLV DDIFTVTEDE IKCATQLVWE RMKLLIEPTA GVGVAAVLSQ HFQTVSPEVK NICIVLSGGN VDLTSSITWV KQAERPASYQ SVSV
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Glycine and serine and threonine metabolism

Gene Information

• Gene Name: SRR serine racemase [ Homo sapiens ]
• Official Symbol: SRR
• Synonyms: SRR; serine racemase; ILV1; ISO1; OTTHUMP00000115699; D-serine dehydratase; L-serine dehydratase; D-serine ammonia-lyase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.17; EC 4.3.1.18
• Gene ID: 63826
• mRNA Refseq: NM_021947
• Protein Refseq: NP_068766
• MIM: 606477
• UniProt ID: Q9GZT4
• Chromosome Location: 17p13
• Function: ATP binding; D-serine ammonia-lyase activity; L-serine ammonia-lyase activity; PDZ domain binding; calcium ion binding; glycine binding; isomerase activity; lyase activity; magnesium ion binding; nucleotide binding; protein homodimerization activity; pyridoxal phosphate binding; serine racemase activity; threonine racemase activity