Recombinant Human serine peptidase inhibitor, Kazal type 1, His-tagged

• Cat.No.: SPINK1-243H
• Product Overview: SPINK1, 24-79aa, Human, His tag, E.coli

Specification

• Description: SPINK1 is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. Recombinant human SPINK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 8.6 kDa (79aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSDSLGREA KCYNELNGCT KIYDPVCGTD GNTYPNECVL CFENRKRQTS ILIQKSGPC
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Protein length: 24-79

Gene Information

• Gene Name: SPINK1 serine peptidase inhibitor, Kazal type 1 [ Homo sapiens ]
• Official Symbol: SPINK1
• Synonyms: SPINK1; serine peptidase inhibitor, Kazal type 1; serine protease inhibitor, Kazal type 1; pancreatic secretory trypsin inhibitor; PCTT; PSTI; Spink3; TATI; tumor-associated trypsin inhibitor; serine protease inhibitor Kazal-type 1; TCP
• Gene ID: 6690
• mRNA Refseq: NM_003122
• Protein Refseq: NP_003113
• MIM: 167790
• UniProt ID: P00995
• Chromosome Location: 5q32
• Function: endopeptidase inhibitor activity; protein binding; serine-type endopeptidase inhibitor activity