Recombinant Human SEMG1 Protein, His-tagged

• Cat.No.: SEMG1-221H
• Product Overview: Recombinant Human SEMG1, transcript variant 2, fused with His tag at C-terminal was expressed in HEK296 cells.

Specification

• Description: The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. This preproprotein is proteolytically processed by the prostate-specific antigen (PSA) protease to generate multiple peptide products that exhibit distinct functions. One of these peptides, SgI-29, is an antimicrobial peptide with antibacterial activity. This proteolysis process also breaks down the gel matrix and allows the spermatozoa to move more freely. This gene and another similar semenogelin gene are present in a gene cluster on chromosome 20.
• Source: HEK293 cells
• Tag: His
• Form: Lyophilized from a 0.2 µM filtered solution of 20mM Hac-NaAc, 150mM NaCl, pH 4.5
• Molecular Mass: 43.8kD
• AA Sequence: QKGGSKGRLPSEFSQFPHGQKGQHYSGQKGKQQTESKGSFSIQYTYHVDANDHDQSRKSQQYDLNALHKTTKSQRHLGGSQQLLHNKQEGRDHDKSKGHFHRVVIHHKGGKAHRGTQNPSQDQGNSPSGKGISSQYSNTEERLWVHGLSKEQTSVSGAQKGRKQGGSQSSYVLQTEELVANKQQRETKNSHQNKGHYQNVVEVREEHSSKVQTSLCPAHQDKLQHGSKDIFSTQDELLVYNKNQHQTKNLNQDQQHGRKANKISYQSSSTEERRLHYGENGVQKDVSQRSIYSQTEKLVAGKSQIQAPNPKQEPWHGENAKGESGQSTNREQDLLSHEQKGRHQHGSHGGLDIVIIEQEDDSDRHLAQHLNNDQNPLFTVDHHHHHH
• Endotoxin: Endotoxin level is <0.1 ng/µg of protein (<1EU/µg).
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Gene Information

• Gene Name: SEMG1 semenogelin I [ Homo sapiens ]
• Official Symbol: SEMG1
• Synonyms: SEMG1; semenogelin I; SEMG; semenogelin-1; cancer/testis antigen 103; CT103; semen coagulating protein; SGI; dJ172H20.2; FLJ78262; MGC14719
• Gene ID: 6406
• mRNA Refseq: NM_003007
• Protein Refseq: NP_002998
• MIM: 182140
• UniProt ID: P04279