Recombinant Human SEMA3F protein, His-tagged

• Cat.No.: SEMA3F-1913H
• Product Overview: Recombinant Human SEMA3F aa. (Val350~Leu483) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin loop and a C-terminal basic domain. This gene is expressed by the endothelial cells where it was found to act in an autocrine fashion to induce apoptosis, inhibit cell proliferation and survival, and function as an anti-tumorigenic agent. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Val350~Leu483
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 9.2

Gene Information

• Gene Name: SEMA3F semaphorin 3F [ Homo sapiens (human) ]
• Official Symbol: SEMA3F
• Synonyms: SEMA3F; semaphorin 3F; SEMA4; SEMAK; SEMA-IV; semaphorin-3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F; semaphorin III/F; semaphorin IV
• Gene ID: 6405
• mRNA Refseq: NM_001318800.1
• Protein Refseq: NP_001305729.1
• UniProt ID: Q13275