Recombinant Human SELP protein, His-Avi-tagged, Biotinylated

• Cat.No.: SELP-109HB
• Product Overview: Biotinylated Recombinant Human SELP protein(P16109)(Trp42-Ala771), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Trp42-Ala771
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% saccharose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human P-Selectin, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 82.9 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: SELP selectin P (granule membrane protein 140kDa, antigen CD62) [ Homo sapiens ]
• Official Symbol: SELP
• Synonyms: SELP; selectin P (granule membrane protein 140kDa, antigen CD62); GRMP, selectin P (granule membrane protein 140kD, antigen CD62); P-selectin; CD62; CD62P; GMP140; PADGEM; PSEL; GMP-140; granule membrane protein 140; granulocyte membrane protein; CD62 antigen-like family member P; platelet alpha-granule membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein; GRMP; LECAM3; FLJ45155
• Gene ID: 6403
• mRNA Refseq: NM_003005
• Protein Refseq: NP_002996
• UniProt ID: P16109